Differential expression of small RNAs from Burkholderia thailandensis in response to varying environmental and stress conditions.

ABSTRACT:

Background

Bacterial small RNAs (sRNAs) regulate gene expression by base-pairing with downstream target mRNAs to attenuate translation of mRNA into protein at the post-transcriptional level. In response to specific environmental changes, sRNAs can modulate the expression levels of target genes, thus enabling adaptation of cellular physiology.

Results

We profiled sRNA expression in the Gram-negative bacteria Burkholderia thailandensis cultured under 54 distinct growth conditions using a Burkholderia-specific microarray that contains probe sets to all intergenic regions greater than 90 bases. We identified 38 novel sRNAs and performed experimental validation on five sRNAs that play a role in adaptation of Burkholderia to cell stressors. In particular, the trans-encoded BTH_s1

SUBMITTER: Stubben CJ 

PROVIDER: S-EPMC4035088 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature