Macrophages promote progression of spasmolytic polypeptide-expressing metaplasia after acute loss of parietal cells.

ABSTRACT:

Background & aims

Loss of parietal cells causes the development of spasmolytic polypeptide-expressing metaplasia (SPEM) through transdifferentiation of chief cells. In the presence of inflammation, SPEM can advance into a more proliferative metaplasia with increased expression of intestine-specific transcripts. We used L635 to induce acute SPEM with inflammation in mice and investigated the roles of inflammatory cells in the development of SPEM.

Methods

To study the adaptive immune system, Rag1 knockout, interferon-γ-deficient, and wild-type (control) mice received L635 for 3 days. To study the innate immune system, macrophages were depleted by intraperitoneal injection of clodronate liposomes 2 days before and throughout L635 administration. Neutrophils were depleted by int

SUBMITTER: Petersen CP 

PROVIDER: S-EPMC4035414 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature