Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics.

ABSTRACT: Using high-resolution MS-based proteomics in combination with multiple protease digestion, we profiled, with on average 90% sequence coverage, all 13 viral proteins present in an human adenovirus (HAdV) vector. This in-depth profile provided multiple peptide-based evidence on intrinsic protease activity affecting several HAdV proteins. Next, the generated peptide library was used to develop a targeted proteomics method using selected reaction monitoring (SRM) aimed at quantitative profiling of the stoichiometry of all 13 proteins present in the HAdV. We also used this method to probe the release of specific virus proteins initiated by thermal stimulation, mimicking the early stage of HAdV disassembly during entry into host cells. We confirmed the copy numbers of the most well characterized

SUBMITTER: Benevento M 

PROVIDER: S-EPMC4036278 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature