Fluorescent labelling of the actin cytoskeleton in plants using a cameloid antibody.

ABSTRACT:

Background

Certain members of the Camelidae family produce a special type of antibody with only one heavy chain. The antigen binding domains are the smallest functional fragments of these heavy-chain only antibodies and as a consequence have been termed nanobodies. Discovery of these nanobodies has allowed the development of a number of therapeutic proteins and tools. In this study a class of nanobodies fused to fluorescent proteins (chromobodies), and therefore allowing antigen-binding and visualisation by fluorescence, have been used. Such chromobodies can be expressed in living cells and used as genetically encoded immunocytochemical markers.

Results

Here a modified version of the commercially available Actin-Chromobody® as a novel tool for visualising actin dynamics in t

SUBMITTER: Rocchetti A 

PROVIDER: S-EPMC4036722 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature