Defining the full tomato NB-LRR resistance gene repertoire using genomic and cDNA RenSeq.

ABSTRACT:

Background

The availability of draft crop plant genomes allows the prediction of the full complement of genes that encode NB-LRR resistance gene homologs, enabling a more targeted breeding for disease resistance. Recently, we developed the RenSeq method to reannotate the full NB-LRR gene complement in potato and to identify novel sequences that were not picked up by the automated gene prediction software. Here, we established RenSeq on the reference genome of tomato (Solanum lycopersicum) Heinz 1706, using 260 previously identified NB-LRR genes in an updated Solanaceae RenSeq bait library.

Result

Using 250-bp MiSeq reads after RenSeq on genomic DNA of Heinz 1706, we identified 105 novel NB-LRR sequences. Reannotation included the splitting of gene models, combination of part

SUBMITTER: Andolfo G 

PROVIDER: S-EPMC4036795 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature