EST mining and functional expression assays identify extracellular effector proteins from the plant pathogen Phytophthora.

ABSTRACT: Plant pathogenic microbes have the remarkable ability to manipulate biochemical, physiological, and morphological processes in their host plants. These manipulations are achieved through a diverse array of effector molecules that can either promote infection or trigger defense responses. We describe a general functional genomics approach aimed at identifying extracellular effector proteins from plant pathogenic microorganisms by combining data mining of expressed sequence tags (ESTs) with virus-based high-throughput functional expression assays in plants. PexFinder, an algorithm for automated identification of extracellular proteins from EST data sets, was developed and applied to 2147 ESTs from the oomycete plant pathogen Phytophthora infestans. The program identified 261 ESTs (12.2%) cor

SUBMITTER: Torto TA 

PROVIDER: S-EPMC403741 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature