Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells.

ABSTRACT: Molecular markers defining self-renewing pluripotent embryonic stem cells (ESCs) have been identified by relative comparisons between undifferentiated and differentiated cells. Most of analysis has been done under a specific differentiation condition that may present significantly different molecular changes over others. Therefore, it is currently unclear if there are true consensus markers defining undifferentiated human ESCs (hESCs). To identify a set of key genes consistently altered during differentiation of hESCs regardless of differentiation conditions, we have performed microarray analysis on undifferentiated hESCs (H1 and H9) and differentiated EBs and validated our results using publicly available expression array datasets. We constructed consensus modules by Weighted Gene Coexpre

SUBMITTER: Kim JJ 

PROVIDER: S-EPMC4037450 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature