Ontology highlight
ABSTRACT:
SUBMITTER: Boley N
PROVIDER: S-EPMC4037530 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Nature biotechnology 20140316 4
The identification of full length transcripts entirely from short-read RNA sequencing data (RNA-seq) remains a challenge in the annotation of genomes. Here we describe an automated pipeline for genome annotation that integrates RNA-seq and gene-boundary data sets, which we call Generalized RNA Integration Tool, or GRIT. Applying GRIT to Drosophila melanogaster short-read RNA-seq, cap analysis of gene expression (CAGE) and poly(A)-site-seq data collected for the modENCODE project, we recovered th ...[more]