Detection of a divergent Parainfluenza 4 virus in an adult patient with influenza like illness using next-generation sequencing.

ABSTRACT:

Background

Human Parainfluenza viruses are a common cause of both upper and lower respiratory tract infections, particularly in children. Of the four Parainfluenza virus serotypes, Parainfluenza 4 is least well characterised from both the clinical, epidemiological and genetic perspectives.

Methods

Flocked nose or throat swabs from a previous study investigating viral prevalence in community-based adults suffering from influenza like illness were used as the basis for this study. Samples in which no virus was detected using a 16 viral respiratory pathogen real-time PCR panel were barcoded and pyrosequenced using the Roche 454 GS FLX Titanium chemistry. The sequences were analysed using the VirusHunter bioinformatic pipeline. Sanger sequencing was used to complete the detected

SUBMITTER: Bialasiewicz S 

PROVIDER: S-EPMC4038074 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature