Ectopic integration vectors for generating fluorescent promoter fusions in Bacillus subtilis with minimal dark noise.

ABSTRACT: Fluorescent protein promoter reporters are important tools that are widely used for diverse purposes in microbiology, systems biology and synthetic biology and considerable engineering efforts are still geared at improving the sensitivity of the reporter systems. Here we focus on dark noise, i.e. the signal that is generated by the empty vector control. We quantitatively characterize the dark noise of a few common bacterial reporter systems by single cell microscopy. All benchmarked reporter systems generated significant amounts of dark noise that exceed the cellular autofluorescence to different extents. We then reengineered a multicolor set of fluorescent ectopic integration vectors for Bacillus subtilis by introducing a terminator immediately upstream of the promoter insertion site, res

SUBMITTER: Trauth S 

PROVIDER: S-EPMC4038550 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature