Lectin-carbohydrate interactions on nanoporous gold monoliths.

ABSTRACT: Monoliths of nanoporous gold (np-Au) were modified with self-assembled monolayers of octadecanethiol (C₁₈-SH), 8-mercaptooctyl α-D-mannopyranoside (αMan-C₈-SH), and 8-mercapto-3,6-dioxaoctanol (HO-PEG₂-SH), and the loading was assessed using thermogravimetric analysis (TGA). Modification with mixed SAMs containing αMan-C₈-SH (at a 0.20 mole fraction in the SAM forming solution) with either octanethiol or HO-PEG₂-SH was also investigated. The np-Au monoliths modified with αMan-C₈-SH bind the lectin Concanavalin A (Con A), and the additional mass due to bound protein was assessed using TGA analysis. A comparison of TGA traces measured before and after exposure of HO-PEG₂-SH modified np-Au to Con A showed that the no

SUBMITTER: Tan YH 

PROVIDER: S-EPMC4038695 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature