Genetic variability and in vitro transcriptional permissibility of primary ovine beta-retrovirus promoter isolates.

ABSTRACT:

Objective

To assess genomic sequence conservation and variation in the proviral promoter of enzootic nasal tumor virus (ENTV) and Jaagsiekte sheep retrovirus (JSRV) in tissue samples from 3 sheep with nasal adenocarcinoma associated with ENTV and 3 sheep with pulmonary adenocarcinoma associated with JSRV and to identify a cell culture system that supports transcriptional activity of the ENTV and JSRV viral promoters.

Animals

6 adult sheep.

Procedures

Standard PCR procedures for detection of the ENTV and JSRV long terminal repeat (LTR) promoter region were performed on samples from the 3 nasal adenocarcinomas and 3 pulmonary adenocarcinomas, respectively. The LTRs were cloned into shuttle vectors, amplified, sequenced, and analyzed. The cloned LTR regions were transfer

SUBMITTER: Eckstrand CD 

PROVIDER: S-EPMC4038715 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature