A viral over-expression system for the major malaria mosquito Anopheles gambiae.

ABSTRACT: Understanding pathogen/mosquito interactions is essential for developing novel strategies to control mosquito-borne diseases. Technical advances in reverse-genetics, such as RNA interference (RNAi), have facilitated elucidation of components of the mosquito immune system that are antagonistic to pathogen development, and host proteins essential for parasite development. Forward genetic approaches, however, are limited to generation of transgenic insects, and while powerful, mosquito transgenesis is a resource- and time-intensive technique that is not broadly available to most laboratories. The ability to easily "over-express" genes would enhance molecular studies in vector biology and expedite elucidation of pathogen-refractory genes without the need to make transgenic insects. We develope

SUBMITTER: Suzuki Y 

PROVIDER: S-EPMC4038844 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature