KRAS, NRAS and BRAF mutations in Greek and Romanian patients with colorectal cancer: a cohort study.

ABSTRACT:

Objectives

Treatment decision-making in colorectal cancer is often guided by tumour tissue molecular analysis. The aim of this study was the development and validation of a high-resolution melting (HRM) method for the detection of KRAS, NRAS and BRAF mutations in Greek and Romanian patients with colorectal cancer and determination of the frequency of these mutations in the respective populations.

Setting

Diagnostic molecular laboratory located in Athens, Greece.

Participants

2425 patients with colorectal cancer participated in the study.

Primary and secondary outcome measures

2071 patients with colorectal cancer (1699 of Greek and 372 of Romanian origin) were analysed for KRAS exon 2 mutations. In addition, 354 tumours from consecutive patients (196 Greek and 1

SUBMITTER: Negru S 

PROVIDER: S-EPMC4039802 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature