Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering.

ABSTRACT: Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

SUBMITTER: Wei L 

PROVIDER: S-EPMC4040164 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature