Uncoated endocytic vesicles require the unconventional myosin, Myo6, for rapid transport through actin barriers.

ABSTRACT: After clathrin-mediated endocytosis, clathrin removal yields an uncoated vesicle population primed for fusion with the early endosome. Here we present the first characterization of uncoated vesicles and show that myo6, an unconventional myosin, functions to move these vesicles out of actin-rich regions found in epithelial cells. Time-lapse microscopy revealed that myo6-associated uncoated vesicles were motile and exhibited fusion and stretching events before endosome delivery, processes that were dependent on myo6 motor activity. In the absence of myo6 motor activity, uncoated vesicles remained trapped in the actin mesh, where they exhibited Brownian-like motion. Exit from the actin mesh occurred by a slow diffusion-based mechanism, delaying transferrin trafficking to the early endosome. E

SUBMITTER: Aschenbrenner L 

PROVIDER: S-EPMC404020 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature