Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis.

ABSTRACT:

Background

Plasmacytoid dendritic cells have been implicated in the pathogenesis of systemic sclerosis through mechanisms beyond the previously suggested production of type I interferon.

Methods

We isolated plasmacytoid dendritic cells from healthy persons and from patients with systemic sclerosis who had distinct clinical phenotypes. We then performed proteome-wide analysis and validated these observations in five large cohorts of patients with systemic sclerosis. Next, we compared the results with those in patients with systemic lupus erythematosus, ankylosing spondylitis, and hepatic fibrosis. We correlated plasma levels of CXCL4 protein with features of systemic sclerosis and studied the direct effects of CXCL4 in vitro and in vivo.

Results

Proteome-wide analysis

SUBMITTER: van Bon L 

PROVIDER: S-EPMC4040466 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature