Downregulation of microRNA-182-5p contributes to renal cell carcinoma proliferation via activating the AKT/FOXO3a signaling pathway.

ABSTRACT:

Background

Emerging evidence has suggested that dysregulation of miR-182-5p may contribute to tumor development and progression in several types of human cancers. However, its role in renal cell carcinoma (RCC) is still unknown.

Methods

Quantitative RT-PCR was used to quantify miR-182-5p expression in RCC clinical tissues. Bisulfite sequencing PCR was used for DNA methylation analysis. The CCK-8, colony formation, flow cytometry, and a xenograft model were performed. Immunohistochemistry was conducted using the peroxidase and DAB methods. A miR-182-5p target was determined by luciferase reporter assays, quantitative RT-PCR, and Western blotting.

Results

miR-182-5p is frequently down-regulated in human RCC tissues. Epigenetic modulation may be involved in the regulatio

SUBMITTER: Xu X 

PROVIDER: S-EPMC4040501 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature