Telomere length kinetics assay (TELKA) sorts the telomere length maintenance (tlm) mutants into functional groups.

ABSTRACT: Genome-wide systematic screens in yeast have uncovered a large gene network (the telomere length maintenance network or TLM), encompassing more than 400 genes, which acts coordinatively to maintain telomere length. Identifying the genes was an important first stage; the next challenge is to decipher their mechanism of action and to organize then into functional groups or pathways. Here we present a new telomere-length measuring program, TelQuant, and a novel assay, telomere length kinetics assay, and use them to organize tlm mutants into functional classes. Our results show that a mutant defective for the relatively unknown MET7 gene has the same telomeric kinetics as mutants defective for the ribonucleotide reductase subunit Rnr1, in charge of the limiting step in dNTP synthesis, or for t

SUBMITTER: Rubinstein L 

PROVIDER: S-EPMC4041441 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature