Direct squencing from the minimal number of DNA molecules needed to fill a 454 picotiterplate.

ABSTRACT: The large amount of DNA needed to prepare a library in next generation sequencing protocols hinders direct sequencing of small DNA samples. This limitation is usually overcome by the enrichment of such samples with whole genome amplification (WGA), mostly by multiple displacement amplification (MDA) based on φ29 polymerase. However, this technique can be biased by the GC content of the sample and is prone to the development of chimeras as well as contamination during enrichment, which contributes to undesired noise during sequence data analysis, and also hampers the proper functional and/or taxonomic assignments. An alternative to MDA is direct DNA sequencing (DS), which represents the theoretical gold standard in genome sequencing. In this work, we explore the possibility of sequencing th

SUBMITTER: Dzunkova M 

PROVIDER: S-EPMC4041646 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature