Digital gene-expression profiling analysis of the cholesterol-lowering effects of alfalfa saponin extract on laying hens.

ABSTRACT:

Background

To prevent cardiovascular disease, people are advised to limit their intake of dietary cholesterol to less than 300 mg/day. Egg consumption has been seriously reduced because of the high levels of cholesterol. The purpose of the present study was to evaluate the cholesterol-lowering effects of alfalfa saponin extract (ASE) in yolk and the molecular mechanisms underlying these effects using digital gene-expression profiling analysis. Liver and ovary tissues were isolated from laying hens fed with ASE for RNA sequencing.

Results

The cholesterol content of the yolks of eggs from hens fed 120 mg/kg ASE declined considerably on day 60. Other groups (60, 240, 480 mg/kg ASE group) also showed decreases, but they were not significant. Digital gene expression generated ove

SUBMITTER: Zhou L 

PROVIDER: S-EPMC4041749 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature