Characterization of centromeric histone H3 (CENH3) variants in cultivated and wild carrots (Daucus sp.).

ABSTRACT: In eukaryotes, centromeres are the assembly sites for the kinetochore, a multi-protein complex to which spindle microtubules are attached at mitosis and meiosis, thereby ensuring segregation of chromosomes during cell division. They are specified by incorporation of CENH3, a centromere specific histone H3 variant which replaces canonical histone H3 in the nucleosomes of functional centromeres. To lay a first foundation of a putative alternative haploidization strategy based on centromere-mediated genome elimination in cultivated carrots, in the presented research we aimed at the identification and cloning of functional CENH3 genes in Daucus carota and three distantly related wild species of genus Daucus varying in basic chromosome numbers. Based on mining the carrot transcriptome followed

SUBMITTER: Dunemann F 

PROVIDER: S-EPMC4041860 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature