A Nonpolycationic Fully Proteinaceous Multiagent System for Potent Targeted Delivery of siRNA.

ABSTRACT: Protein-based methods of targeted short-interfering RNA (siRNA) delivery have the potential to solve some of the problems faced by nanoparticle-based methods, such as poor pharmacokinetics and biodistribution, low tumor penetration, and polydispersity. However, protein-based targeted delivery has been limited to fusion proteins with polycationic peptides as siRNA carriers, whose high charge density in some cases results in undesirable biophysical and in vivo properties. Here, we present a fully proteinaceous, multiagent approach for targeted siRNA delivery to epidermal growth factor receptor (EGFR), using a nonpolycationic carrier for siRNA. Each agent contributes a fundamentally different mechanism of action that work together for potent targeted RNA interference. The first agent is an EG

SUBMITTER: Liu DV 

PROVIDER: S-EPMC4041992 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature