Concentration and purification of enterovirus 71 using a weak anion-exchange monolithic column.

ABSTRACT:

Background

Enterovirus 71 (EV-71) is a neurotropic virus causing Hand, Foot and Mouth Disease (HFMD) in infants and children under the age of five. It is a major concern for public health issues across Asia-Pacific region. The most effective way to control the disease caused by EV-71 is by vaccination thus a novel vaccine is urgently needed. Inactivated EV-71 induces a strong, virus-neutralizing antibody response in animal models, protecting them against a lethal EV-71 challenge and it has been shown to elicit cross-neutralizing antibodies in human trials. Hence, the large-scale production of purified EV-71 is required for vaccine development, diagnosis and clinical trials.

Methods

CIM® Monolith columns are single-piece columns made up of poly(glycidyl methacrylate co-ethyle

SUBMITTER: Kattur Venkatachalam AR 

PROVIDER: S-EPMC4042139 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature