Dissecting childhood asthma with nasal transcriptomics distinguishes subphenotypes of disease.

ABSTRACT:

Background

Bronchial airway expression profiling has identified inflammatory subphenotypes of asthma, but the invasiveness of this technique has limited its application to childhood asthma.

Objectives

We sought to determine whether the nasal transcriptome can proxy expression changes in the lung airway transcriptome in asthmatic patients. We also sought to determine whether the nasal transcriptome can distinguish subphenotypes of asthma.

Methods

Whole-transcriptome RNA sequencing was performed on nasal airway brushings from 10 control subjects and 10 asthmatic subjects, which were compared with established bronchial and small-airway transcriptomes. Targeted RNA sequencing nasal expression analysis was used to profile 105 genes in 50 asthmatic subjects and 50 control s

SUBMITTER: Poole A 

PROVIDER: S-EPMC4043390 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature