In vivo immunostaining of hemocyte compartments in Drosophila for live imaging.

ABSTRACT: In recent years, Drosophila melanogaster has become an attractive model organism in which to study the structure and development of the cellular immune components. The emergence of immunological markers greatly accelerated the identification of the immune cells (hemocytes), while the creation of genetic reporter constructs allowed unique insight into the structural organization of hematopoietic tissues. However, investigation of the hemocyte compartments by the means of immunological markers requires dissection and fixation, which regularly disrupt the delicate structure and hamper the microanatomical characterization. Moreover, the investigation of transgenic reporters alone can be misleading as their expression often differs from the native expression pattern of their respective genes. W

SUBMITTER: Csordas G 

PROVIDER: S-EPMC4043501 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature