Enhanced specialized transduction using recombineering in Mycobacterium tuberculosis.

ABSTRACT:

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G: enetic engineering has contributed greatly to our understanding of Mycobacterium tuberculosis biology and has facilitated antimycobacterial and vaccine development. However, methods to generate M. tuberculosis deletion mutants remain labor-intensive and relatively inefficient. Here, methods are described that significantly enhance the efficiency (greater than 100-fold) of recovering deletion mutants by the expression of mycobacteriophage recombineering functions during the course of infection with specialized transducing phages delivering allelic exchange substrates. This system has been successfully applied to the CDC1551 strain of M. tuberculosis, as well as to a ΔrecD mutant generated in the CDC1551 parental strain. The latter studies were undertaken as there were

SUBMITTER: Tufariello JM 

PROVIDER: S-EPMC4045075 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature