Protein phosphorylation differs significantly among ontogenetic phases in Malus seedlings.

ABSTRACT:

Background

Although protein phosphorylation is an important post-translational modification affecting protein function and metabolism, dynamic changes in this process during ontogenesis remain unexplored in woody angiosperms.

Methods

Phosphorylated proteins from leaves of three apple seedlings at juvenile, adult vegetative and reproductive stages were extracted and subjected to alkaline phosphatase pre-treatment. After separating the proteins by two-dimensional gel electrophoresis and phosphoprotein-specific Pro-Q Diamond staining, differentially expressed phosphoproteins were identified by MALDI-TOF-TOF mass spectrometry.

Results

A total of 107 phosphorylated protein spots on nine gels (three ontogenetic phases × three seedlings) were identified by MALDI-TOF-TOF mass

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4046019 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature