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HPV16 infection of HaCaTs is dependent on ?4 integrin, and ?6 integrin processing.

ABSTRACT: Our understanding of human papillomavirus (HPV) is still evolving. To further study the field, our laboratory has focused on determining the role of integrins in the initial steps of viral endocytosis into HaCaT cells. Our and others' previous findings have shown that ?6 is necessary for infection. Here we show that ?3 and ?1 were dispensable, and we identified integrin ?6?4 complex as necessary for infection in HaCaTs. ?4 knock down resulted in a significant decrease in HPV16 PsV infection and perhaps most importantly resulted in defective post-translational ?6 processing. We showed that the unprocessed ?6 does not localize to the cell surface. We propose that the ?6?4 complex is necessary for the formation of an endocytic complex that results in the signaling transduction events necessary for initial endocytosis.

SUBMITTER: Aksoy P

PROVIDER: S-EPMC4046336 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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HPV16 infection of HaCaTs is dependent on β4 integrin, and α6 integrin processing.

Aksoy Pınar P Abban Cynthia Y CY Kiyashka Elizabeth E Qiang Weitao W Meneses Patricio I PI

Virology 20131125

Our understanding of human papillomavirus (HPV) is still evolving. To further study the field, our laboratory has focused on determining the role of integrins in the initial steps of viral endocytosis into HaCaT cells. Our and others' previous findings have shown that α6 is necessary for infection. Here we show that α3 and β1 were dispensable, and we identified integrin α6β4 complex as necessary for infection in HaCaTs. β4 knock down resulted in a significant decrease in HPV16 PsV infection and ...[more]

PMID: 24418536

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