Substrate-dependent gene regulation of self-assembled human MSC spheroids on chitosan membranes.

ABSTRACT:

Background

Three-dimensional (3D) multicellular spheroids of mesenchymal stem cells (MSCs) are generally regarded to have beneficial properties over MSCs in monolayer. Recent literatures have documented that MSCs can self-assemble into 3D spheroids with a greater capacity for differentiation into various cell types when grown on chitosan (CS), a biopolymer. The genomic modulation occurring in these MSC spheroids is thus of essential importance for understanding their uniqueness and therapeutic potentials. In this study, 3D spheroids self-assembled from human umbilical cord MSCs grown on CS membranes were analyzed by mRNA as well as microRNA microarrays, which helped identify the critical signaling events that may alter the cellular functions during the spheroid forming process.

SUBMITTER: Yeh HY 

PROVIDER: S-EPMC4046657 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature