Simultaneous analysis of multiple staphylococcal enterotoxin genes by an oligonucleotide microarray assay.

ABSTRACT: Staphylococcal enterotoxins (SEs) are a family of 17 major serological types of heat-stable enterotoxins that are one of the leading causes of gastroenteritis resulting from consumption of contaminated food. SEs are considered potential bioweapons. Many Staphylococcus aureus isolates contain multiple SEs. Because of the large number of SEs, serological typing and PCR typing are laborious and time-consuming. Furthermore, serological typing may not always be practical because of antigenic similarities among enterotoxins. We report on a microarray-based one-tube assay for the simultaneous detection and identification (genetic typing) of multiple enterotoxin (ent) genes. The proposed typing method is based on PCR amplification of the target region of the ent genes with degenerate primers, foll

SUBMITTER: Sergeev N 

PROVIDER: S-EPMC404666 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature