Pseudo-Sanger sequencing: massively parallel production of long and near error-free reads using NGS technology.

ABSTRACT:

Background

Usually, next generation sequencing (NGS) technology has the property of ultra-high throughput but the read length is remarkably short compared to conventional Sanger sequencing. Paired-end NGS could computationally extend the read length but with a lot of practical inconvenience because of the inherent gaps. Now that Illumina paired-end sequencing has the ability of read both ends from 600 bp or even 800 bp DNA fragments, how to fill in the gaps between paired ends to produce accurate long reads is intriguing but challenging.

Results

We have developed a new technology, referred to as pseudo-Sanger (PS) sequencing. It tries to fill in the gaps between paired ends and could generate near error-free sequences equivalent to the conventional Sanger reads in length but

SUBMITTER: Ruan J 

PROVIDER: S-EPMC4046676 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature