Kinetic mechanism at the branchpoint between the DNA synthesis and editing pathways in individual DNA polymerase complexes.

ABSTRACT: Exonucleolytic editing of incorrectly incorporated nucleotides by replicative DNA polymerases (DNAPs) plays an essential role in the fidelity of DNA replication. Editing requires that the primer strand of the DNA substrate be transferred between the DNAP polymerase and exonuclease sites, separated by a distance that is typically on the order of ~30 Å. Dynamic transitions between functional states can be quantified with single-nucleotide spatial precision and submillisecond temporal resolution from ionic current time traces recorded when individual DNAP complexes are held atop a nanoscale pore in an electric field. In this study, we have exploited this capability to determine the kinetic relationship between the translocation step and primer strand transfer between the polymerase and exonuc

SUBMITTER: Lieberman KR 

PROVIDER: S-EPMC4046759 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature