Genetic background drives transcriptional variation in human induced pluripotent stem cells.

ABSTRACT: Human iPS cells have been generated using a diverse range of tissues from a variety of donors using different reprogramming vectors. However, these cell lines are heterogeneous, which presents a limitation for their use in disease modeling and personalized medicine. To explore the basis of this heterogeneity we generated 25 iPS cell lines under normalised conditions from the same set of somatic tissues across a number of donors. RNA-seq data sets from each cell line were compared to identify the majority contributors to transcriptional heterogeneity. We found that genetic differences between individual donors were the major cause of transcriptional variation between lines. In contrast, residual signatures from the somatic cell of origin, so called epigenetic memory, contributed relatively

SUBMITTER: Rouhani F 

PROVIDER: S-EPMC4046971 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature