Proteomic analysis of adult Ascaris suum fluid compartments and secretory products.

ABSTRACT:

Background

Strategies employed by parasites to establish infections are poorly understood. The host-parasite interface is maintained through a molecular dialog that, among other roles, protects parasites from host immune responses. Parasite excretory/secretory products (ESP) play major roles in this process. Understanding the biology of protein secretion by parasites and their associated functional processes will enhance our understanding of the roles of ESP in host-parasite interactions.

Methodology/principal findings

ESP was collected after culturing 10 adult female Ascaris suum. Perienteric fluid (PE) and uterine fluid (UF) were collected directly from adult females by dissection. Using SDS-PAGE coupled with LC-MS/MS, we identified 175, 308 and 274 proteins in ESP, PE and

SUBMITTER: Chehayeb JF 

PROVIDER: S-EPMC4046973 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature