Src kinases regulate de novo actin polymerization during exocytosis in neuroendocrine chromaffin cells.

ABSTRACT: The cortical actin network is dynamically rearranged during secretory processes. Nevertheless, it is unclear how de novo actin polymerization and the disruption of the preexisting actin network control transmitter release. Here we show that in bovine adrenal chromaffin cells, both formation of new actin filaments and disruption of the preexisting cortical actin network are induced by Ca2+ concentrations that trigger exocytosis. These two processes appear to regulate different stages of exocytosis; whereas the inhibition of actin polymerization with the N-WASP inhibitor wiskostatin restricts fusion pore expansion, thus limiting the release of transmitters, the disruption of the cortical actin network with cytochalasin D increases the amount of transmitter released per event. Further, the Sr

SUBMITTER: Olivares MJ 

PROVIDER: S-EPMC4047038 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature