Exploring the polyadenylated RNA virome of sweet potato through high-throughput sequencing.

ABSTRACT:

Background

Viral diseases are the second most significant biotic stress for sweet potato, with yield losses reaching 20% to 40%. Over 30 viruses have been reported to infect sweet potato around the world, and 11 of these have been detected in China. Most of these viruses were detected by traditional detection approaches that show disadvantages in detection throughput. Next-generation sequencing technology provides a novel, high sensitive method for virus detection and diagnosis.

Methodology/principal findings

We report the polyadenylated RNA virome of three sweet potato cultivars using a high throughput RNA sequencing approach. Transcripts of 15 different viruses were detected, 11 of which were detected in cultivar Xushu18, whilst 11 and 4 viruses were detected in Guangshu 8

SUBMITTER: Gu YH 

PROVIDER: S-EPMC4047073 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature