Dataset Information

Nrf2 activation protects the liver from ischemia/reperfusion injury in mice.

ABSTRACT:

Objective

To investigate the role of Nrf2 in the pathogenesis of hepatic ischemia-reperfusion (I/R) injury.

Background

Hepatic I/R injury is a serious complication that leads to liver failure after liver surgery. NF-E2-related factor 2 (Nrf2) is a transcription factor that plays a critical role in protecting cells against oxidative stress. Therefore, it is suggested that Nrf2 activation protects the liver from I/R injury.

Methods

Wild-type and Nrf2-deficient mice were treated with 15-deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2), or a vehicle. Subsequently, these mice were subjected to 60-minute hepatic 70% ischemia, followed by reperfusion. Liver and blood samples were collected to evaluate liver injury and mRNA expressions.