Do lipids show state-dependent affinity to the voltage-gated potassium channel KvAP?

ABSTRACT: As all integral membrane proteins, voltage-gated ion channels are embedded in a lipid matrix that regulates their channel behavior either by physicochemical properties or by direct binding. Because manipulation of the lipid composition in cells is difficult, we investigated the influence of different lipids on purified KvAP channels reconstituted in planar lipid bilayers of known composition. Lipids developed two distinct and independent effects on the KvAP channels; lipids interacting with the pore lowered the energy barriers for the final transitions, whereas voltage sensor-bound lipids shifted the midpoint of activation dependent on their electrostatic charge. Above all, the midpoint of activation was determined only by those lipids the channels came in contact with first after purifica

SUBMITTER: Faure E 

PROVIDER: S-EPMC4047412 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature