Structural and functional analysis of novel human cytochrome C targets in apoptosis.

ABSTRACT: Since the first description of apoptosis four decades ago, great efforts have been made to elucidate, both in vivo and in vitro, the molecular mechanisms involved in its regulation. Although the role of cytochrome c during apoptosis is well established, relatively little is known about its participation in signaling pathways in vivo due to its essential role during respiration. To obtain a better understanding of the role of cytochrome c in the onset of apoptosis, we used a proteomic approach based on affinity chromatography with cytochrome c as bait in this study. In this approach, novel cytochrome c interaction partners were identified whose in vivo interaction and cellular localization were facilitated through bimolecular fluorescence complementation. Modeling of the complex interface b

SUBMITTER: Martinez-Fabregas J 

PROVIDER: S-EPMC4047465 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature