A universal chemical enrichment method for mapping the yeast N-glycoproteome by mass spectrometry (MS).

ABSTRACT: Glycosylation is one of the most common and important protein modifications in biological systems. Many glycoproteins naturally occur at low abundances, which makes comprehensive analysis extremely difficult. Additionally, glycans are highly heterogeneous, which further complicates analysis in complex samples. Lectin enrichment has been commonly used, but each lectin is inherently specific to one or several carbohydrates, and thus no single or collection of lectin(s) can bind to all glycans. Here we have employed a boronic acid-based chemical method to universally enrich glycopeptides. The reaction between boronic acids and sugars has been extensively investigated, and it is well known that the interaction between boronic acid and diols is one of the strongest reversible covalent bond inte

SUBMITTER: Chen W 

PROVIDER: S-EPMC4047475 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature