Confetti: a multiprotease map of the HeLa proteome for comprehensive proteomics.

ABSTRACT: Bottom-up proteomics largely relies on tryptic peptides for protein identification and quantification. Tryptic digestion often provides limited coverage of protein sequence because of issues such as peptide length, ionization efficiency, and post-translational modification colocalization. Unfortunately, a region of interest in a protein, for example, because of proximity to an active site or the presence of important post-translational modifications, may not be covered by tryptic peptides. Detection limits, quantification accuracy, and isoform differentiation can also be improved with greater sequence coverage. Selected reaction monitoring (SRM) would also greatly benefit from being able to identify additional targetable sequences. In an attempt to improve protein sequence coverage and to

SUBMITTER: Guo X 

PROVIDER: S-EPMC4047476 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature