Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung.

ABSTRACT:

Unlabelled

Acinetobacter baumannii is a Gram-negative bacterium that causes diseases such as pneumonia, bacteremia, and soft tissue infections in hospitalized patients. Relatively little is known about how A. baumannii causes these infections. Thus, we used insertion sequencing (INSeq), a combination of transposon mutagenesis and massively parallel next-generation sequencing, to identify novel virulence factors of A. baumannii. To this end, we generated a random transposon mutant library containing 150,000 unique insertions in A. baumannii strain ATCC 17978. The INSeq analysis identified 453 genes required for growth in rich medium. The library was then used in a murine pneumonia model, and the relative levels of abundance of mutants before and after selection in the mouse were com

SUBMITTER: Wang N 

PROVIDER: S-EPMC4049102 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature