Human leukocyte antigen haplotype phasing by allele-specific enrichment with peptide nucleic acid probes.

ABSTRACT: Targeted capture of large fragments of genomic DNA that enrich for human leukocyte antigen (HLA) system haplotypes has utility in haematopoietic stem cell transplantation. Current methods of HLA matching are based on inference or familial studies of inheritance; and each approach has its own inherent limitations. We have designed and tested a probe-target-extraction method for capturing specific HLA haplotypes by hybridization of peptide nucleic acid (PNA) probes to alleles of the HLA-DRB1 gene. Short target fragments contained in plasmids were initially used to optimize the method followed by testing samples of genomic DNA from human subjects with preselected HLA haplotypes and obtained approximately 10% enrichment for the specific haplotype. When performed with high-molecular-weight geno

SUBMITTER: Murphy NM 

PROVIDER: S-EPMC4049365 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature