Analysis of differentially expressed genes in two immunologically distinct strains of Eimeria maxima using suppression subtractive hybridization and dot-blot hybridization.

ABSTRACT:

Background

It is well known that different Eimeria maxima strains exhibit significant antigenic variation. However, the genetic basis of these phenotypes remains unclear.

Methods

Total RNA and mRNA were isolated from unsporulated oocysts of E. maxima strains SH and NT, which were found to have significant differences in immunogenicity in our previous research. Two subtractive cDNA libraries were constructed using suppression subtractive hybridization (SSH) and specific genes were further analyzed by dot-blot hybridization and qRT-PCR analysis.

Results

A total of 561 clones were selected from both cDNA libraries and the length of the inserted fragments was 0.25-1.0 kb. Dot-blot hybridization revealed a total of 86 differentially expressed clones (63 from strain SH and

SUBMITTER: Liu D 

PROVIDER: S-EPMC4049472 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature