A variant of green fluorescent protein exclusively deposited to active intracellular inclusion bodies.

ABSTRACT:

Background

Inclusion bodies (IBs) were generally considered to be inactive protein deposits and did not hold any attractive values in biotechnological applications. Recently, some IBs of recombinant proteins were confirmed to show their functional properties such as enzyme activities, fluorescence, etc. Such biologically active IBs are not commonly formed, but they have great potentials in the fields of biocatalysis, material science and nanotechnology.

Results

In this study, we characterized the IBs of DL4, a deletion variant of green fluorescent protein which forms active intracellular aggregates. The DL4 proteins expressed in Escherichia coli were exclusively deposited to IBs, and the IBs were estimated to be mostly composed of active proteins. The spectral properties and

SUBMITTER: Raghunathan G 

PROVIDER: S-EPMC4049505 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature