Evaluation of a pooled strategy for high-throughput sequencing of cosmid clones from metagenomic libraries.

ABSTRACT: High-throughput sequencing methods have been instrumental in the growing field of metagenomics, with technological improvements enabling greater throughput at decreased costs. Nonetheless, the economy of high-throughput sequencing cannot be fully leveraged in the subdiscipline of functional metagenomics. In this area of research, environmental DNA is typically cloned to generate large-insert libraries from which individual clones are isolated, based on specific activities of interest. Sequence data are required for complete characterization of such clones, but the sequencing of a large set of clones requires individual barcode-based sample preparation; this can become costly, as the cost of clone barcoding scales linearly with the number of clones processed, and thus sequencing a large num

SUBMITTER: Lam KN 

PROVIDER: S-EPMC4049660 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature