Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe.

ABSTRACT: The nuclear pore complex (NPC) is an enormous proteinaceous complex composed of multiple copies of about 30 different proteins called nucleoporins. In this study, we analyzed the composition of the NPC in the model organism Schizosaccharomyces pombe using strains in which individual nucleoporins were tagged with GFP. We identified 31 proteins as nucleoporins by their localization to the nuclear periphery. Gene disruption analysis in previous studies coupled with gene disruption analysis in the present study indicates that 15 of these nucleoporins are essential for vegetative cell growth and the other 16 nucleoporins are non-essential. Among the 16 non-essential nucleoporins, 11 are required for normal progression through meiosis and their disruption caused abnormal spore formation or poor

SUBMITTER: Asakawa H 

PROVIDER: S-EPMC4049921 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature