Functional analysis of human Na(+)/K(+)-ATPase familial or sporadic hemiplegic migraine mutations expressed in Xenopus oocytes.

ABSTRACT:

Aim

Functional characterization of ATP1A2 mutations that are related to familial or sporadic hemiplegic migraine (FHM2, SHM).

Methods

cRNA of human Na(+)/K(+)-ATPase α2- and β1-subunits were injected in Xenopus laevis oocytes. FHM2 or SHM mutations of residues located in putative α/β interaction sites or in the α2-subunit's C-terminal region were investigated. Mutants were analyzed by the two-electrode voltage-clamp (TEVC) technique on Xenopus oocytes. Stationary K(+)-induced Na(+)/K(+) pump currents were measured, and the voltage dependence of apparent K(+) affinity was investigated. Transient currents were recorded as ouabain-sensitive currents in Na(+) buffers to analyze kinetics and voltage-dependent pre-steady state charge translocations. The expression of constructs wa

SUBMITTER: Spiller S 

PROVIDER: S-EPMC4050117 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature