Cutting-edge analysis of extracellular microparticles using ImageStream(X) imaging flow cytometry.

ABSTRACT: Interest in extracellular vesicle biology has exploded in the past decade, since these microstructures seem endowed with multiple roles, from blood coagulation to inter-cellular communication in pathophysiology. In order for microparticle research to evolve as a preclinical and clinical tool, accurate quantification of microparticle levels is a fundamental requirement, but their size and the complexity of sample fluids present major technical challenges. Flow cytometry is commonly used, but suffers from low sensitivity and accuracy. Use of Amnis ImageStream(X) Mk II imaging flow cytometer afforded accurate analysis of calibration beads ranging from 1 μm to 20 nm; and microparticles, which could be observed and quantified in whole blood, platelet-rich and platelet-free plasma and in leukocy

SUBMITTER: Headland SE 

PROVIDER: S-EPMC4050385 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature